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Molecular Docking Glossary

Understand the vocabulary of computational molecular docking — from binding affinity and scoring functions to virtual screening.

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Molecular Docking

A computational method that predicts the preferred orientation and binding affinity of a ligand when bound to a protein target.

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Binding Affinity

The strength of interaction between a protein and ligand, typically measured as ΔG (kcal/mol) or Ki/IC50 values. More negative ΔG = stronger binding.

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RMSD

Root Mean Square Deviation — measures the average distance between atoms of superimposed structures. Used to compare docked poses against crystal structures.

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Vina (AutoDock Vina)

A widely-used open-source molecular docking program with improved scoring accuracy and speed over AutoDock4, employing a knowledge-based scoring function.

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GNINA

A deep-learning molecular docking tool that uses 3D convolutional neural networks for scoring, built on the AutoDock Vina framework.

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Virtual Screening

Computational evaluation of large compound libraries against a protein target to identify potential hits, using docking, pharmacophore, or ML-based methods.

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Protein Structure Prediction

Computational methods that predict the 3D structure of a protein from its amino acid sequence, enabling structure-based drug design.

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AlphaFold

DeepMind's AI system that predicts protein structures with near-experimental accuracy, revolutionizing structural biology and drug discovery.

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ESMFold

Meta AI's protein structure prediction model based on large language model embeddings, offering faster predictions without multiple sequence alignments.

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Protein Domain

A conserved, independently-folding region of a protein that often has a specific function, such as ligand binding or protein–protein interaction.

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Protein

A macromolecule composed of amino acid chains that performs enzymatic, structural, and regulatory functions in cells.

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Proteome

The complete set of proteins expressed by a genome, cell, tissue, or organism at a given time under specific conditions.

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IC50

The half-maximal inhibitory concentration — the concentration of a compound that inhibits a biological process by 50%, a key measure of drug potency.

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Drug Discovery

The process of identifying new therapeutic compounds through target identification, hit screening, lead optimization, and preclinical development.

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Pharmacodynamics

The study of how a drug affects the body — the relationship between drug concentration and its pharmacological effect.

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Pharmacokinetics

The study of how the body affects a drug — absorption, distribution, metabolism, and excretion (ADME) over time.

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ADME

Absorption, Distribution, Metabolism, Excretion — the four pharmacokinetic properties that determine a drug's bioavailability and dosing regimen.

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Bioavailability

The fraction of an administered drug that reaches systemic circulation, a critical parameter for oral drug formulation and dosing.

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Lead Compound

A chemical compound that shows promising biological activity and serves as the starting point for optimization into a drug candidate.

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