Everything for primer and PCR work
From a template sequence to ordered oligos: design the pair, check it in silico, then confirm the thermodynamics against published assays.
Primer & Probe Design
24-step pipeline: thermodynamics, BLAST specificity, SNP filter, repeat masking and a multiplex check.
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PCR Analysis
Run both primers against your template for amplicon size, product sequence and match positions.
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PCR Product CalculatorFree
Expected amplicon size and product sequence straight from your two primer sequences.
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Tm Calculator
Nearest-neighbour melting temperature with salt, magnesium and oligo-concentration corrections.
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GC Calculator
GC%, base counts and molecular weight for any DNA or RNA sequence.
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Biostatistics CalculatorFree
t-tests, ANOVA, chi-square, regression, power analysis, EC50/IC50 — 20+ calculators.
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Primer Design Guide
The workflow from template sequence to ordered oligos: parameters, thermodynamics and specificity.
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Thermodynamic Validation
Published primer pairs — N1, GAPDH and ACTB — run through the same engine so you can reproduce the numbers.
Open toolOpen any tool above directly — no installation, no desktop software. Browse all hubs or see what each plan unlocks.